Abstract: Background: In spite huge leaps in combating the spread and transmission of HIV globally, Nigeria still ranks high in the incidence of HIV among children. The study investigates the factors affecting the utilization of prevention of mother-to-child transmission (PMTCT) of HIV services in Anambra South. Data source and Method: Data from 515 HIV positive women of child bearing age were collected using a structured questionnaire. The data sets were subjected to chi square and binary logistic regression analysis. Result: The results show that 86% of the respondents were aware of the PMTCT strategies while marital status (69.8%), age (70.7%) and place of residence (52. I%) were found to affect the uptake and utilization of PMTCT services. Conclusion: Evidence from the study drew attention to the need for government, social worl‹ers and healthcare providers to rise to their responsibilities in encouraging HIV women of reproductive age to utilize PMTCT services in order to achieve African Union Agenda 3. Keywords: Mother-to-Child Transmission, PMTCT Utilization, HIV in Nigeria, Anambra South, Social Worker